CJC-1295 Reconstitution & Dosage Protocol
GHRH(1-29) with Drug Affinity Complex — Complete laboratory preparation guide
Ares Research Lab Team
Analytical Chemistry Division
READ TIME
7 MIN READ
Complete reconstitution, dosage calculation, and storage protocol for CJC-1295 DAC-modified GHRH analog. Covers sterile water preparation, concentration math for somatotroph assays, and long-term stability data.
CAS NUMBER
863288-34-0
MOLECULAR FORMULA
C152H252N42O42
MOLECULAR WEIGHT
3367.97 g/mol
PURITY
≥ 99.6% (HPLC)
SEQUENCE
Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser + DAC
RECONSTITUTION PROCEDURE
All Ares Research CJC-1295 vials arrive as lyophilized powder sealed under inert gas. Reconstitution requires bacteriostatic water to prevent bacterial contamination. Do not use tap water, sterile water, or saline alone.
Temperature Equilibration
Remove vial from -20°C storage and allow 15 minutes at room temperature before reconstitution.
Volume Calculation
For a 10mg vial, inject 10ml bacteriostatic water to achieve 1.0 mg/ml. Use a sterile insulin-grade syringe for accurate measurement.
Slow Injection
Insert needle at 45° angle through rubber stopper. Inject solvent gently along the interior glass wall. Do not inject directly onto the powder cake.
Gentle Agitation
Roll vial between palms for 60 seconds. CJC-1295 dissolves readily. Visual inspection: solution should clarify completely.
Reconstituted CJC-1295 is stable for 7 days at 2–8°C. For long-term storage, aliquot into separate vials and freeze at -20°C immediately. Avoid repeated freeze-thaw cycles.
CONCENTRATION & DOSAGE REFERENCE
Standard Ares Research CJC-1295 vials contain 10mg of lyophilized GHRH analog. The table below shows concentration after reconstitution.
RESEARCH DOSAGE RANGES
- →Somatotroph GH secretion (GH3 / primary pituitary): 0.1–10 mcg/ml in serum-free DMEM/F12, 1–24 hours
- →Hepatocyte IGF-1 upregulation: 0.1–1.0 mcg/ml in HepG2 or primary hepatocyte cultures, 24–72 hours
- →Adipocyte lipolysis assays: 0.01–1.0 mcg/ml in differentiated 3T3-L1 cultures, 6–24 hours
- →DPP-IV resistance validation: pre-incubate with recombinant DPP-IV (50–100 ng/ml) for 2 hours, then assay GH release
STORAGE & STABILITY
CJC-1295 is highly stable in lyophilized form due to its compact 29-aa structure and lack of oxidation-sensitive residues.
- →Lyophilized powder: stable ≥ 36 months at -20°C, protected from light
- →Reconstituted solution: 7 days at 2–8°C; aliquot and freeze at -20°C for long-term storage
- →Aliquots at -20°C: stable ≥ 6 months; thaw each aliquot once and discard after use
- →Protect from UV and fluorescent light exposure at all stages
- →pH of reconstituted solution should remain 6.5–7.5; discard if discoloration or precipitation occurs
ANALYTICAL SPECIFICATIONS
REFERENCES & CITATIONS
1. Teichman, S.L. et al. (2006). Prolonged stimulation of growth hormone release by CJC-1295. Endocrine, 30(2), 201–208.
2. Sackmann-Sala, L. et al. (2009). CJC-1295 increases GH and IGF-I levels. Growth Hormone & IGF Research, 19(4), 380–383.
3. Ionescu, M. & Frohman, L.A. (2006). Pulsatile secretion of growth hormone. Growth Hormone & IGF Research, 16(1), 1–12.
RESEARCH DISCLAIMER
All protocols are intended solely for qualified laboratory research professionals. Not for human use or consumption. For in-vitro and laboratory research only.
— QUICK SPECS
— RELATED PROTOCOLS
REPAIR PEPTIDE
BPC-157
8 MIN READ
REGENERATION PEPTIDE
TB-500
7 MIN READ
COPPER PEPTIDE
GHK-Cu
6 MIN READ
NOOTROPIC PEPTIDE
Semax
6 MIN READ
VIEW COMPOUNDS
Browse research catalog
— FREQUENTLY ASKED QUESTIONS
Q: What water should I use to reconstitute CJC-1295?
Use bacteriostatic water (0.9% benzyl alcohol) exclusively. Sterile water lacks a preservative and allows bacterial growth. Never use tap water, distilled water, or saline without bacteriostatic additives.
Q: How long does reconstituted CJC-1295 last?
Reconstituted CJC-1295 remains stable for approximately 7 days when stored at 2–8°C in the dark. For longer storage, aliquot into separate sterile vials and freeze at -20°C. Avoid freeze-thaw cycling.
Q: What concentration should I use for GH secretion assays?
For GH3 rat pituitary somatotrophs or primary anterior pituitary cell cultures, CJC-1295 is typically evaluated at 0.1–10 mcg/ml in serum-free DMEM/F12. GH secretion is measured by radioimmunoassay or ELISA at 1, 3, 6, and 24 hours. Always include a vehicle control.
Q: Does the DAC modification affect reconstitution?
The DAC (Drug Affinity Complex) is a C-terminal modification that does not significantly alter solubility or reconstitution behavior. CJC-1295 dissolves readily in bacteriostatic water within 60 seconds. The DAC group becomes biologically active only after intravenous or subcutaneous administration in in-vivo models.
Q: Is CJC-1295 the same as Tesamorelin?
No. CJC-1295 is a 29-amino-acid truncated GHRH analog with DAC modification and DPP-IV resistance. Tesamorelin is a 44-amino-acid full-length GHRH analog with trans-3-hexenoic acid conjugation. They differ in sequence length, half-life, stability mechanism, and research applications.
ARES RESEARCH
RESEARCH CART (0)
CART IS EMPTY
Add research compounds from the catalog to begin your inquiry.
RESEARCH USE ONLY. All compounds are strictly for in-vitro laboratory research by qualified professionals. Not for human consumption, veterinary use, or clinical application.